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Search results: ASK1 MAP3K5 MEKK5

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Western blot (WB) analysis of ASK1 antibody at 1/500 dilution Lane 1:HEK293T cell lysate Lane 2:PC12 cell lysate Lane 3:Mouse heart tissue lysate
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyAP06018PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
Immunohistochemistry (IHC) analyzes of ASK1 antibody (Cat.-No.: AP21014PU-N) in paraffin-embedded human breast carcinoma tissue.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyAP21014PU-N
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetMAP3K5
  • SizePrice
Immunohistochemistry (IHC) analyzes of ASK1 antibody (Cat.-No.: AP21015PU-N) in paraffin-embedded human lung adenocarcinoma tissue.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyAP21015PU-N
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP3K5
  • SizePrice
Western blot analysis of extracts of various cell lines, using MAP3K5 antibody (TA378269) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 30s.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA378269
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
Western blot analysis of extracts of various cell lines, using MAP3K5 antibody (TA378270) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 10s.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA378270
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP3K5
  • SizePrice
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA321734 (MAP3K5 Antibody) at dilution 1/20 (Original magnification: x200)
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA321734
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAP3K5 (Cat# RC209913, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K5 antibody (Cat# TA890021). Positive lysates [LY401794] (100ug) and [LC401794] (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA890021
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP3K5
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAP3K5 (Cat# RC209913, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K5 antibody (Cat# TA890021). Positive lysates [LY401794] (100ug) and [LC401794] (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA890021S
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP3K5
  • SizePrice
Western blot analysis of extracts of A-431 and HeLa cells, using Phospho-MAP3K5-S1033 antibody (TA378271) at 1:1000 dilution. A431 cells were treated by PMA/TPA (200nM). HeLa cells were treated by 10% FBS or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA378271
ApplicationsWestern Blot
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot analysis of extracts of various cell lines, using Phospho-MAP3K5-S83 antibody (TA378272) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour, treated by TNF-alpha (20ng/mL) for 30 minutes after serum-starvation overnight or treated by ATP (5mM) at 30°C for 1 hour. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 60s.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA378272
ApplicationsWestern Blot
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot analysis of extracts of HeLa cells, using Phospho-MAP3K5-T918 antibody (TA378273) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA378273
ApplicationsWestern Blot
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot analysis of extracts of Jurkat cells, using Phospho-MAP3K5-S967 antibody (TA378275) at 1:1000 dilution.Jurkat cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA378275
ApplicationsWestern Blot
ReactivityHuman
TargetMAP3K5
  • SizePrice
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