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Figure 1. Western blot analysis of Apg3 using anti-Apg3 antibody (A01768-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apg3 antigen affinity purified polyclonal antibody (Catalog # A01768-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apg3 at approximately 36-40 kDa. The expected band size for Apg3 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-Apg3/ATG3 Antibody Picoband(r)A01768-1-CARRIER-FREE
ApplicationsWestern Blot
TargetATG3
  • SizePrice
Figure 1. Western blot analysis of Apg3 using anti-Apg3 antibody (A01768-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apg3 antigen affinity purified polyclonal antibody (Catalog # A01768-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apg3 at approximately 36-40 kDa. The expected band size for Apg3 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-Apg3/ATG3 Antibody Picoband(r)A01768-1-FITC
ApplicationsWestern Blot
TargetATG3
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, Apg3/ATG3 cDNA ORF Clone, C-His tagHG15302-CH
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, Apg3/ATG3 cDNA ORF Clone, C-Myc tagHG15302-CM
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, Apg3/ATG3 cDNA ORF Clone, C-HA tagHG15302-CY
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, Apg3/ATG3 cDNA ORF Clone in Cloning VectorHG15302-G
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, Apg3/ATG3 cDNA ORF Clone, N-His tagHG15302-NH
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, Apg3/ATG3 cDNA ORF Clone, N-Myc tagHG15302-NM
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Mouse, Apg3/ATG3 cDNA ORF Clone, N-Myc tagMG53262-NM
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Human, Apg3/ATG3 cDNA ORF Clone, N-HA tagHG15302-NY
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Rat, Apg3/ATG3 cDNA ORF Clone in Cloning VectorRG84922-U
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Mouse, Apg3/ATG3 cDNA ORF Clone, C-His tagMG53262-CH
CategoryDNA / RNA / Vectors
  • SizePrice
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