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Search results: BAF CRISPR

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LAG3 CRISPR/Cas9 Lentivirus (Integrating)
Product group Molecular Biology
BPS Bioscience
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TIGIT CRISPR/Cas9 Lentivirus (Integrating)
Product group Molecular Biology
BPS Bioscience
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PD-1 CRISPR/Cas9 Lentivirus (Integrating)
Product group Molecular Biology
BPS Bioscience
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Figure 1. Western blot analysis of BCL6 using anti-BCL6 antibody (M00142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL6 antigen affinity purified monoclonal antibody (Catalog # M00142-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL6 at approximately 95 kDa. The expected band size for BCL6 is at 79 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetBCL6
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Product group Genome Editing and Engineering
Applied Biological Materials
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Product group Genome Editing and Engineering
Santa Cruz Biotechnology
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Product group Genome Editing and Engineering
Santa Cruz Biotechnology
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Product group Genome Editing and Engineering
Santa Cruz Biotechnology
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Product group Genome Editing and Engineering
Santa Cruz Biotechnology
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Product group Genome Editing and Engineering
Santa Cruz Biotechnology
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Product group Genome Editing and Engineering
Santa Cruz Biotechnology
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Product group Genome Editing and Engineering
Santa Cruz Biotechnology
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