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Figure 1. Western blot analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/BCL2L11 antigen affinity purified polyclonal antibody (Catalog # A01552-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/BCL2L11 at approximately 28 kDa. The expected band size for Bim/BCL2L11 is at 22 kDa.
Product group Antibodies
Boster Bio
Anti-Bim/BCL2L11 Antibody Picoband(r)A01552-4-FITC
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetBCL2L11
  • SizePrice
Figure 1. Western blot analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/BCL2L11 antigen affinity purified polyclonal antibody (Catalog # A01552-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/BCL2L11 at approximately 28 kDa. The expected band size for Bim/BCL2L11 is at 22 kDa.
Product group Antibodies
Boster Bio
Anti-Bim/BCL2L11 Antibody Picoband(r)A01552-4-HRP
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetBCL2L11
  • SizePrice
Figure 1. Western blot analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/BCL2L11 antigen affinity purified polyclonal antibody (Catalog # A01552-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/BCL2L11 at approximately 28 kDa. The expected band size for Bim/BCL2L11 is at 22 kDa.
Product group Antibodies
Boster Bio
Anti-Bim/BCL2L11 Antibody Picoband(r)A01552-4-IFLUOR647
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetBCL2L11
  • SizePrice
Figure 1. Western blot analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/BCL2L11 antigen affinity purified polyclonal antibody (Catalog # A01552-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/BCL2L11 at approximately 28 kDa. The expected band size for Bim/BCL2L11 is at 22 kDa.
Product group Antibodies
Boster Bio
Anti-Bim/BCL2L11 Antibody Picoband(r)A01552-4-PE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetBCL2L11
  • SizePrice
Figure 1. Western blot analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/BCL2L11 antigen affinity purified polyclonal antibody (Catalog # A01552-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/BCL2L11 at approximately 28 kDa. The expected band size for Bim/BCL2L11 is at 22 kDa.
Product group Antibodies
Boster Bio
Anti-Bim/BCL2L11 Antibody Picoband(r)A01552-4
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetBCL2L11
  • SizePrice
Product group Assays
Biomatik
Bim ELISA KitEKA50063
Assay Sample TypeResearchers plate their cell line of choice
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Chemicals
Biorbyt
BIM-23190 hydrochlorideORB1693157
Estimated Purity98%
  • SizePrice
Product group Chemicals
Biorbyt
BIM-23190 aceateORB1303554
Estimated Purity99.70%
Molecular Weight1262.5
  • SizePrice
Anti-BIM (phospho Ser59) Antibody
Product group Antibodies
Antibodies.com
Anti-BIM (phospho Ser59) AntibodyA94327
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Anti-BIM (Phospho-Ser69) Antibody
Product group Antibodies
Antibodies.com
Anti-BIM (Phospho-Ser69) AntibodyA51414
ApplicationsImmunoFluorescence, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Anti-BIM (Ab-69) Antibody
Product group Antibodies
Antibodies.com
Anti-BIM (Ab-69) AntibodyA36557
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Bim BH3 [505070-06-4]
Product group Chemicals
TargetMol Chemicals
Bim BH3 [505070-06-4]T80038
Molecular Weight2444.77
  • SizePrice
1011121314

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