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Search results: CCND3

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Western blot analysis of Cyclin D3 expression in K562 cell lysate (M01744). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCND3 monoclonal antibody (Catalog # M01744) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCND3
Product group Antibodies
Boster Bio
Anti-Cyclin D3 CCND3 Rabbit Monoclonal AntibodyM01744
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetCCND3
Product group Antibodies
Biorbyt
Cyclin D3 (phospho-T283) antibodyORB338829
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCCND3
  • SizePrice
Western blot analysis of Cyclin D3 using anti-Cyclin D3 antibody (PB9103). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human Cyclin D3 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D3 antigen affinity purified polyclonal antibody (Catalog # PB9103) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D3 at approximately 25KD. The expected band size for Cyclin D3 is at 25KD.
Product group Antibodies
References
Boster Bio
Anti-Cyclin D3/CCND3 Antibody Picoband(r)PB9103
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetCCND3
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35203 Cyclin D3 antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
Cyclin D3 antibody - KO/KD ValidatedGTX35203
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetCCND3
  • SizePrice
WB analysis of extracts from mouse heart tissue using GTX55411 Cyclin D3 (phospho Thr283) antibody. Left : Primary antibody pre-incubated with the antigen specific peptide Right : Primary antibody
Product group Antibodies
GeneTex
Cyclin D3 (phospho Thr283) antibodyGTX55411
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetCCND3
  • SizePrice
Product group Antibodies
References
Nordic-MUbio
Mouse anti Human Cyclin D3C135M
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCND3
Product group Antibodies
Biorbyt
Cyclin D3 antibody (Biotin)ORB447807
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
TargetCCND3
  • SizePrice
Product group Antibodies
Biorbyt
Cyclin D3 (Phospho-Thr283) antibodyORB156505
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetCCND3
  • SizePrice
Product group Antibodies
Biorbyt
phospho-Cyclin D3 Thr283 antibody (PE)ORB505122
ApplicationsFlow Cytometry, ImmunoCytoChemistry
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetCCND3
  • SizePrice
Product group Antibodies
Biorbyt
phospho-Cyclin D3 Thr283 antibody (HRP)ORB503569
ApplicationsELISA, ImmunoCytoChemistry
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetCCND3
  • SizePrice
Product group Antibodies
Biorbyt
Cyclin D3 antibody (PE)ORB485482
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
TargetCCND3
  • SizePrice
Product group Antibodies
Biorbyt
Cyclin D3 antibody (HRP)ORB469603
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
TargetCCND3
  • SizePrice
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