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Search results: CD134

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Anti-CD134/OX40 antibody, PA2136-2, Western blotting Lane 1: Mouse Brain Tissue Lysate Lane 2: Mouse Spleen Tissue Lysate Lane 3: Mouse Liver Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityMouse
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-2, Western blotting Lane 1: Mouse Brain Tissue Lysate Lane 2: Mouse Spleen Tissue Lysate Lane 3: Mouse Liver Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityMouse
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-2, Western blotting Lane 1: Mouse Brain Tissue Lysate Lane 2: Mouse Spleen Tissue Lysate Lane 3: Mouse Liver Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityMouse
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-2, Western blotting Lane 1: Mouse Brain Tissue Lysate Lane 2: Mouse Spleen Tissue Lysate Lane 3: Mouse Liver Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityMouse
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-2, Western blotting Lane 1: Mouse Brain Tissue Lysate Lane 2: Mouse Spleen Tissue Lysate Lane 3: Mouse Liver Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityMouse
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-2, Western blotting Lane 1: Mouse Brain Tissue Lysate Lane 2: Mouse Spleen Tissue Lysate Lane 3: Mouse Liver Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityMouse
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-2, Western blotting Lane 1: Mouse Brain Tissue Lysate Lane 2: Mouse Spleen Tissue Lysate Lane 3: Mouse Liver Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityMouse
TargetTnfrsf4
  • SizePrice
Product group Antibodies
Assay Genie
ApplicationsFlow Cytometry
ReactivityMouse
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsWestern Blot
ReactivityBovine, Canine, Equine, Human, Mouse, Rat
TargetTNFRSF4
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsWestern Blot
ReactivityBovine, Canine, Equine, Human, Mouse, Rat
TargetTNFRSF4
  • SizePrice
Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetTnfrsf4
  • SizePrice
Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetTnfrsf4
  • SizePrice