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Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
Anti-CD134/OX40/Tnfrsf4 Antibody Picoband(r)A02495-CY3
ApplicationsWestern Blot, ELISA
TargetTnfrsf4
  • SizePrice
Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
Anti-CD134/OX40/Tnfrsf4 Antibody Picoband(r)A02495-DYLIGHT488
ApplicationsWestern Blot, ELISA
TargetTnfrsf4
  • SizePrice
Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
Anti-CD134/OX40/Tnfrsf4 Antibody Picoband(r)A02495-DYLIGHT550
ApplicationsWestern Blot, ELISA
TargetTnfrsf4
  • SizePrice
Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
Anti-CD134/OX40/Tnfrsf4 Antibody Picoband(r)A02495-DYLIGHT594
ApplicationsWestern Blot, ELISA
TargetTnfrsf4
  • SizePrice
Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
Anti-CD134/OX40/Tnfrsf4 Antibody Picoband(r)A02495-FITC
ApplicationsWestern Blot, ELISA
TargetTnfrsf4
  • SizePrice
Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
Anti-CD134/OX40/Tnfrsf4 Antibody Picoband(r)A02495-HRP
ApplicationsWestern Blot, ELISA
TargetTnfrsf4
  • SizePrice
Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
Anti-CD134/OX40/Tnfrsf4 Antibody Picoband(r)A02495-IFLUOR647
ApplicationsWestern Blot, ELISA
TargetTnfrsf4
  • SizePrice
Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
Anti-CD134/OX40/Tnfrsf4 Antibody Picoband(r)A02495-PE
ApplicationsWestern Blot, ELISA
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-3, Western blotting All lanes: Anti CD134/OX40 (PA2136-3) at 0.5ug/ml Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Rat Liver Tissue Lysate at 50ug Lane 3: Rat Kidney Tissue Lysate at 50ug Predicted bind size: 29KD Observed bind size: 29KD
Product group Antibodies
Boster Bio
Anti-CD134/OX40/TNFRSF4 Antibody Picoband(r)PA2136-3-BIOTIN
ApplicationsWestern Blot
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-3, Western blotting All lanes: Anti CD134/OX40 (PA2136-3) at 0.5ug/ml Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Rat Liver Tissue Lysate at 50ug Lane 3: Rat Kidney Tissue Lysate at 50ug Predicted bind size: 29KD Observed bind size: 29KD
Product group Antibodies
Boster Bio
Anti-CD134/OX40/TNFRSF4 Antibody Picoband(r)PA2136-3-CARRIER-FREE
ApplicationsWestern Blot
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-3, Western blotting All lanes: Anti CD134/OX40 (PA2136-3) at 0.5ug/ml Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Rat Liver Tissue Lysate at 50ug Lane 3: Rat Kidney Tissue Lysate at 50ug Predicted bind size: 29KD Observed bind size: 29KD
Product group Antibodies
Boster Bio
Anti-CD134/OX40/TNFRSF4 Antibody Picoband(r)PA2136-3-CY3
ApplicationsWestern Blot
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-3, Western blotting All lanes: Anti CD134/OX40 (PA2136-3) at 0.5ug/ml Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Rat Liver Tissue Lysate at 50ug Lane 3: Rat Kidney Tissue Lysate at 50ug Predicted bind size: 29KD Observed bind size: 29KD
Product group Antibodies
Boster Bio
Anti-CD134/OX40/TNFRSF4 Antibody Picoband(r)PA2136-3-DYLIGHT488
ApplicationsWestern Blot
TargetTnfrsf4
  • SizePrice
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