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Search results: CD134

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Figure 1. Western blot analysis of CD134 using anti-CD134 antibody (PB9898). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates, Lane 2: 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134 antigen affinity purified polyclonal antibody (Catalog # PB9898) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134 at approximately 50KD. The expected band size for CD134 is at 50KD.
Product group Antibodies
Boster Bio
Anti-CD134/OX40/TNFRSF4 Antibody Picoband(r)PB9898
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTNFRSF4
  • SizePrice
Product group Antibodies
Biorbyt
CD134 Rabbit mAbORB1727257
ApplicationsFlow Cytometry, Western Blot
TargetTNFRSF4
  • SizePrice
Product group Antibodies
Biorbyt
CD134 Rabbit pAbORB1061909
ApplicationsWestern Blot
TargetTNFRSF4
  • SizePrice
Product group Proteins / Signaling Molecules
KACTUS
Human OX40/TNFRSF4/CD134 Protein (OX4-HM240)OX4-HM240
Protein IDP43489
  • SizePrice
Product group Proteins / Signaling Molecules
KACTUS
Human OX40/TNFRSF4/CD134 Protein (OX4-HM440)OX4-HM440
Protein IDP43489
  • SizePrice
Product group Proteins / Signaling Molecules
KACTUS
Mouse OX40/TNFRSF4/CD134 Protein (OX4-MM440)OX4-MM440
Protein IDP47741
  • SizePrice
Product group Antibodies
MyBioSource
Anti-Rat CD134, Purified (Clone OX40) (mouse IgG2b)MBS520049
  • SizePrice
Product group Antibodies
MyBioSource
Anti-Rat CD134, FITC (Clone OX40) (mouse IgG2b)MBS520563
  • SizePrice
Product group Antibodies
MyBioSource
Anti-Rat CD134, Biotin (Clone OX40) (mouse IgG2b)MBS520604
  • SizePrice
Product group Antibodies
MyBioSource
Anti-Rat CD134, PE (Clone OX40) (mouse IgG2b)MBS520621
  • SizePrice
Product group Antibodies
Biorbyt
CD134 Antibody HRP ConjugatedORB1542519
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetTNFRSF4
  • SizePrice
Product group Antibodies
Biorbyt
CD134 Rat monoclonal antibodyORB1495644
ApplicationsFlow Cytometry, ImmunoFluorescence
TargetTnfrsf4
  • SizePrice
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