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Search results: CD134

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Anti-CD134/OX40 antibody, PA2136-1, Western blotting Lane 1: U87 Cell Lysate Lane 2: HELA Cell Lysate Lane 3: HT1080 Cell Lysate Lane 4: JURKAT Cell Lysate Lane 5: COLO320 Cell Lysate Lane 6: MCF-7 Cell Lysate Lane 7: SHC Cell Lysate Lane 8: COLO320 Cell Lysate Lane 9: SGC Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman
TargetTNFRSF4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-2, Western blotting Lane 1: Mouse Brain Tissue Lysate Lane 2: Mouse Spleen Tissue Lysate Lane 3: Mouse Liver Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityMouse
TargetTnfrsf4
  • SizePrice
Anti-CD134/OX40 antibody, PA2136-3, Western blotting All lanes: Anti CD134/OX40 (PA2136-3) at 0.5ug/ml Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Rat Liver Tissue Lysate at 50ug Lane 3: Rat Kidney Tissue Lysate at 50ug Predicted bind size: 29KD Observed bind size: 29KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetTnfrsf4
  • SizePrice
Figure 1. Western blot analysis of CD134 using anti-CD134 antibody (PB9898). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates, Lane 2: 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134 antigen affinity purified polyclonal antibody (Catalog # PB9898) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134 at approximately 50KD. The expected band size for CD134 is at 50KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTNFRSF4
  • SizePrice

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