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Search results: CDC25B

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Figure 1. Western blot analysis of CDC25B using anti-CDC25B antibody (A01899-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human Hacat whole cell lysates. Lane 7: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25B antigen affinity purified polyclonal antibody (Catalog # A01899-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC25B at approximately 70 kDa. The expected band size for CDC25B is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-CDC25B Antibody Picoband(r)A01899-1-10UG
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Figure 1. Western blot analysis of CDC25B using anti-CDC25B antibody (A01899-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human Hacat whole cell lysates. Lane 7: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25B antigen affinity purified polyclonal antibody (Catalog # A01899-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC25B at approximately 70 kDa. The expected band size for CDC25B is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-CDC25B Antibody Picoband(r)A01899-1-APC
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Figure 1. Western blot analysis of Cdc25b using anti-Cdc25b antibody (A01899). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25b antigen affinity purified polyclonal antibody (Catalog # A01899) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25b at approximately 65 kDa. The expected band size for Cdc25b is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-Cdc25b Antibody Picoband(r)A01899-10UG
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetCdc25b
  • SizePrice
Figure 1. Western blot analysis of Cdc25b using anti-Cdc25b antibody (A01899). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25b antigen affinity purified polyclonal antibody (Catalog # A01899) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25b at approximately 65 kDa. The expected band size for Cdc25b is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-Cdc25b Antibody Picoband(r)A01899-APC
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetCdc25b
  • SizePrice
Product group Chemicals
MedChem Express
CDC25B-IN-2 [134271-74-2]HY-137175A
CAS Number134271-74-2
Estimated Purity97.02
Molecular Weight268.27
  • SizePrice
CDC25B-IN-2 [134271-74-2]
Product group Chemicals
TargetMol Chemicals
CDC25B-IN-2 [134271-74-2]T9156
CAS Number134271-74-2
Estimated Purity99.12%
Molecular Weight268.27
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B AntibodyLS-C309848
ApplicationsWestern Blot
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B AntibodyLS-C402687
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B AntibodyLS-C417491
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B AntibodyLS-C417492
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B AntibodyLS-C801584
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCDC25B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CDC25B AntibodyLS-C664504
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCDC25B
  • SizePrice
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