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Search results: CDC37

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Western blot analysis of Phospho-CDC37 (S13) expression in (1) Jurkat cell lysate treated with Alkaline Phosphatase; (2) Jurkat cell lysate (P02169). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC37 monoclonal antibody (Catalog # P02169) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC37
Product group Antibodies
Boster Bio
Product group DNA / RNA / Vectors
Sino Biological
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Product group DNA / RNA / Vectors
Sino Biological
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Product group Lysates / Extracts
Sino Biological
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Product group Proteins / Signaling Molecules
Sino Biological
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Product group Lysates / Extracts
Sino Biological
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Product group Lysates / Extracts
Sino Biological
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Untreated (-) and treated (+) 293T whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC37 (phospho Ser13) antibody [GT1338] (GTX03250) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
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Anti-Cdc37 antibody, PA2139, Western blotting All lanes: Anti Cdc37(PA2139) at 0.5ug/ml Lane 1: JURKAT Whole Cell Lysate at 40ug Lane 2: 293T Whole Cell Lysate at 40ug Predicted bind size: 44KD Observed bind size: 44KD
Product group Antibodies
Boster Bio
Product group Antibodies
Biorbyt
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Product group Antibodies
Biorbyt
ApplicationsELISA
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Product group Antibodies
Biorbyt
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