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Search results: CDC37

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Figure 1. Western blot analysis of Cdc37 using anti-Cdc37 antibody (PB9574). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc37 antigen affinity purified polyclonal antibody (Catalog # PB9574) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc37 at approximately 44 kDa. The expected band size for Cdc37 is at 44 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman
TargetCDC37
  • SizePrice
Figure 1. Western blot analysis of Cdc37 using anti-Cdc37 antibody (PB9574). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc37 antigen affinity purified polyclonal antibody (Catalog # PB9574) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc37 at approximately 44 kDa. The expected band size for Cdc37 is at 44 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman
TargetCDC37
  • SizePrice
Figure 1. Western blot analysis of Cdc37 using anti-Cdc37 antibody (PB9574). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc37 antigen affinity purified polyclonal antibody (Catalog # PB9574) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc37 at approximately 44 kDa. The expected band size for Cdc37 is at 44 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman
TargetCDC37
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
  • SizePrice
Anti-p50 CDC37 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Anti-Cdc37 (H9) Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Anti-Cdc37 (F259) Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice