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Search results: DDAH1

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Product group Antibodies
LifeSpan BioSciences
DDAH1 AntibodyLS-C816223
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
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Product group Assays
DLdevelop
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Product group Assays
DLdevelop
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GTX68337-pro Image
Product group Molecular Biology
GeneTex
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Product group Proteins / Signaling Molecules
GeneTex
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Immunohistochemical staining of human kidney shows high expression.
Product group Antibodies
Atlas Antibodies
Anti-DDAH1HPA006308
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDDAH1
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Product group Antibodies
Atlas Antibodies
Anti-DDAH1HPA071064
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetDDAH1
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Product group Antibodies
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
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Western blot analysis of DDAH1 expression in 293 cell lysate using anti-DDAH1 antibody (M03136). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH1 antigen affinity purified polyclonal antibody (Catalog # M03136) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for DDAH1.
Product group Antibodies
Boster Bio
Western blot analysis of DDAH1 using anti-DDAH1 antibody (PB10000). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH1 antigen affinity purified polyclonal antibody (Catalog # PB10000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDAH1 at approximately 31 KD, 37KD. The expected band size for DDAH1 is at 31KD.
Product group Antibodies
Boster Bio
GTX89709 WB Image
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Chicken, Drosophila, Equine, Guinea Pig, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus
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