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Search results: DHFR

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DHFR-IN-3 [137553-43-6]
Product group Chemicals
TargetMol Chemicals
DHFR-IN-3 [137553-43-6]T3693
CAS Number137553-43-6
Estimated Purity99.61%
Molecular Weight239.07
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Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (M00813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.
Product group Antibodies
References
Boster Bio
Anti-DHFR Antibody (monoclonal, 3C8)M00813-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetDHFR
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Product group Antibodies
LifeSpan BioSciences
DHFR Antibody (HRP)LS-C545662
ApplicationsWestern Blot
ReactivityHuman
TargetDHFR
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Product group Antibodies
LifeSpan BioSciences
DHFR Antibody (APC)LS-C516401
ApplicationsWestern Blot
ReactivityHuman
TargetDHFR
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Product group Antibodies
LifeSpan BioSciences
DHFR Antibody (Biotin)LS-C525544
ApplicationsWestern Blot
ReactivityHuman
TargetDHFR
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Product group Antibodies
LifeSpan BioSciences
DHFR Antibody (FITC)LS-C535560
ApplicationsWestern Blot
ReactivityHuman
TargetDHFR
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Product group Antibodies
LifeSpan BioSciences
DHFR Antibody (PE)LS-C653741
ApplicationsWestern Blot
ReactivityHuman
TargetDHFR
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Product group Chemicals
MedChem Express
EcDHFR/FKBP12 F36V binder-1HY-159758
Molecular Weight1009.15
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Product group Antibodies
Bioss
DHFR Recombinant Antibody, PerCP ConjugatedBSM-61547R-PERCP
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
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Product group Antibodies
Bioss
DHFR Polyclonal Antibody, PerCP ConjugatedBS-9058R-PERCP
ApplicationsImmunoFluorescence
ReactivityHuman, Mouse
TargetDHFR
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Product group Molecular Biology
Sino Biological
Human, Dihydrofolate reductase (DHFR) cDNA ORF Clone, C-His tagHG14365-CH
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Product group Molecular Biology
Sino Biological
Human, Dihydrofolate reductase (DHFR) cDNA ORF Clone, C-Myc tagHG14365-CM
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