Bio-Connect

Search results: EMP2

445 to 456 of 1.451 products
Sort by
Product group Antibodies
Cusabio
TMED7 AntibodyCSB-PA896886XA01HU
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetTMED7
  • SizePrice
WB analysis of THP-1 cells using GTX45128 TMED8 antibody at 0.2-1microg/ml.
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman
TargetTMED8
  • SizePrice
WB analysis of Jurkat cells using GTX45130 TMED8 antibody at 0.2-1microg/ml.
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman
TargetTMED8
  • SizePrice
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTMED8
  • SizePrice
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTMED7
  • SizePrice
Product group Antibodies
Cusabio
TMED5 AntibodyCSB-PA023661XA01HU
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetTMED5
  • SizePrice