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Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-TMED5/p28 Antibody Picoband(r)A12319-1-DYLIGHT488
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-TMED5/p28 Antibody Picoband(r)A12319-1-DYLIGHT550
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-TMED5/p28 Antibody Picoband(r)A12319-1-DYLIGHT594
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-TMED5/p28 Antibody Picoband(r)A12319-1-FITC
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-TMED5/p28 Antibody Picoband(r)A12319-1-HRP
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-TMED5/p28 Antibody Picoband(r)A12319-1-IFLUOR647
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-TMED5/p28 Antibody Picoband(r)A12319-1-PE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetTMED5
  • SizePrice
Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-TMED5/p28 Antibody Picoband(r)A12319-1
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetTMED5
  • SizePrice
Western blot All lanes: TMED9 antibody at 4.5microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Lane 3: Human placenta tissue Lane 4: A549 whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa
Product group Antibodies
Cusabio
TMED9 AntibodyCSB-PA04804A0RB
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetTMED9
  • SizePrice
Product group Antibodies
Cusabio
TMED9 Antibody, HRP conjugatedCSB-PA04804B0RB
ApplicationsELISA
ReactivityHuman
TargetTMED9
  • SizePrice
Product group Antibodies
Cusabio
TMED9 Antibody, FITC conjugatedCSB-PA04804C0RB
ReactivityHuman
TargetTMED9
  • SizePrice
Product group Antibodies
Cusabio
TMED9 Antibody, Biotin conjugatedCSB-PA04804D0RB
ApplicationsELISA
ReactivityHuman
TargetTMED9
  • SizePrice
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