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Search results: EMP2

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Product group Proteins / Signaling Molecules
MedChem Express
Protein IDQ9BVK6
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Product group Proteins / Signaling Molecules
MedChem Express
Protein IDQ9BVK6
  • SizePrice
Product group Proteins / Signaling Molecules
Sino Biological
Protein IDQ13445
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Product group Proteins / Signaling Molecules
Sino Biological
Protein IDQ13445
  • SizePrice
Product group Proteins / Signaling Molecules
MedChem Express
Protein IDQ13445
  • SizePrice
Product group Proteins / Signaling Molecules
MedChem Express
Protein IDQ13445
  • SizePrice
Product group Proteins / Signaling Molecules
MedChem Express
Protein IDQ7Z7H5
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Figure 1. Western blot analysis of TMED4 using anti-TMED4 antibody (A12647-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED4 antigen affinity purified polyclonal antibody (Catalog # A12647-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED4 at approximately 25 kDa. The expected band size for TMED4 is at 26 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTMED4
  • SizePrice
Figure 1. Western blot analysis of TMED4 using anti-TMED4 antibody (A12647-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED4 antigen affinity purified polyclonal antibody (Catalog # A12647-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED4 at approximately 25 kDa. The expected band size for TMED4 is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-TMED4 Antibody Picoband(r)A12647-1-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTMED4
  • SizePrice
Figure 1. Western blot analysis of TMED4 using anti-TMED4 antibody (A12647-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED4 antigen affinity purified polyclonal antibody (Catalog # A12647-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED4 at approximately 25 kDa. The expected band size for TMED4 is at 26 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTMED4
  • SizePrice
Figure 1. Western blot analysis of TMED4 using anti-TMED4 antibody (A12647-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED4 antigen affinity purified polyclonal antibody (Catalog # A12647-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED4 at approximately 25 kDa. The expected band size for TMED4 is at 26 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTMED4
  • SizePrice
Figure 1. Western blot analysis of TMED4 using anti-TMED4 antibody (A12647-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED4 antigen affinity purified polyclonal antibody (Catalog # A12647-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED4 at approximately 25 kDa. The expected band size for TMED4 is at 26 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTMED4
  • SizePrice