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Search results: ERK2 MAPK1

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Western blot analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF (P00104-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody (Catalog # P00104-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK3
Product group Antibodies
References
Boster Bio
Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) MAPK3 Rabbit Monoclonal AntibodyP00104-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMAPK3
Product group Antibodies
OriGene
ERK1 / ERK2 / ERK3 / ERK4 Rabbit Polyclonal AntibodyAP55088SU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Western blot (WB) analysis of ERK1/2 (pTyr204) antibody (Cat.-No.: AP01886PU-N) in extracts from HeLa cells treated with PMA.
Product group Antibodies
OriGene
ERK1 / ERK2 pTyr204 Rabbit Polyclonal AntibodyAP01886PU-N
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Western blot (WB) analyzes of p-ERK 1/2 antibody (Cat.-No.: AP20845PU-N)
Product group Antibodies
OriGene
ERK1 / ERK2 pThr202 Rabbit Polyclonal AntibodyAP20845PU-N
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
Western blot analysis of Hela cell lysis using MAPK1-3 antibody
Product group Antibodies
OriGene
ERK1 / ERK2 (N-term) Rabbit Polyclonal AntibodyAP23276PU-N
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
ERK2 (1-360, His-tag) Human Protein
Product group Proteins / Signaling Molecules
OriGene
ERK2 (1-360, His-tag) Human ProteinAR09328PU-L
Protein IDP28482
  • SizePrice
ERK2 (1-360, His-tag) Human Protein
Product group Proteins / Signaling Molecules
OriGene
ERK2 (1-360, His-tag) Human ProteinAR09328PU-N
Protein IDP28482
  • SizePrice
Product group Antibodies
Arigo Biolaboratories
anti-ERK1 phospho (Thr202) + ERK2 phospho (Thr185) antibodyARG40680
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMAPK3
  • SizePrice
Product group Antibodies
Bioss Antibodies
ERK1(T202/Y204)+ERK2(T183/Y185) AntBS-1646R-A647
Product group Proteins / Signaling Molecules
MyBioSource
ERK2 Protein, Mouse, Recombinant (His & GST Tag)MBS8122743
Protein IDP63085
  • SizePrice
Product group Lysates / Extracts
MyBioSource
Mouse ERK2 Baculovirus-Insect cells Overexpression LysateMBS8116609
  • SizePrice
Product group Lysates / Extracts
MyBioSource
Mouse ERK2 Baculovirus-Insect cells Overexpression LysateMBS8116610
  • SizePrice
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