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Search results: ESM1

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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityMouse
TargetESM1
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
TargetESM1
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityMouse
TargetESM1
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityMouse
TargetESM1
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityRat
TargetESM1
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman
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Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityMouse
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Product group Antibodies
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
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Western blot analysis of ESM1 using anti-ESM1 antibody (A04169-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human ESM1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESM1 antigen affinity purified polyclonal antibody (Catalog # A04169-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESM1 at approximately 25KD. The expected band size for ESM1 is at 20KD.
Product group Antibodies
Boster Bio
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman
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Product group Proteins / Signaling Molecules
Elabscience
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Product group Proteins / Signaling Molecules
Elabscience
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