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Western blot analysis of TMUB2 Antibody (Center) in mouse kidney tissue lysates (35 ug/lane). TMUB2 (arrow) was detected using the purified Pab.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetTMUB2
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
Anti-WDR46 Antibody Picoband(r)A12816-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice
Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetWDR46
  • SizePrice