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HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GRK5 (Cat# RC209137, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRK5 antibody(Cat# TA890122). Positive lysates [LY401636] (100ug) and [LC401636] (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice
Western blot analysis of extracts from HUVEC cells, HepG2 cells and HeLa cells, using GRK5 antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice
Figure 2. IHC analysis of GRK5 using anti-GRK5 antibody (PB9708). GRK5 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-GRK5 Antibody (PB9708) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Mouse, Rat
TargetGRK5
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
GRK 5 (D-9) ACSC-518005AC
ApplicationsImmunoPrecipitation
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
GRK 5 (D-9) FITCSC-518005FITC
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
GRK 5 (D-9) HRPSC-518005HRP
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
GRK 5 (D-9) PESC-518005PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGRK5
  • SizePrice