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Search results: GAA

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Western blot analysis of extracts of various cell lines, using GAA antibody.
Product group Antibodies
Boster Bio
Anti-Lysosomal alpha-glucosidase GAA AntibodyA01548-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
TargetGAA
  • SizePrice
Product group Antibodies
Bioss
GAA Recombinant Antibody, APC-Cy7 ConjugatedBSM-54735R-APC-CY7
ApplicationsFlow Cytometry, Western Blot
TargetGAA
  • SizePrice
Product group Antibodies
Bioss
GAA Recombinant Antibody, PE-Cy5 ConjugatedBSM-54735R-PE-CY5
ApplicationsFlow Cytometry, Western Blot
TargetGAA
  • SizePrice
Product group Antibodies
Bioss
GAA Recombinant Antibody, PE-Cy7 ConjugatedBSM-54735R-PE-CY7
ApplicationsFlow Cytometry, Western Blot
TargetGAA
  • SizePrice
Product group Antibodies
Bioss
GAA Recombinant Antibody, APC-Cy7 ConjugatedBSM-62357R-APC-CY7
ApplicationsWestern Blot
TargetGAA
  • SizePrice
Product group Antibodies
Bioss
GAA Recombinant Antibody, PE-Cy5 ConjugatedBSM-62357R-PE-CY5
ApplicationsWestern Blot
TargetGAA
  • SizePrice
Product group Antibodies
Bioss
GAA Recombinant Antibody, PE-Cy7 ConjugatedBSM-62357R-PE-CY7
ApplicationsWestern Blot
TargetGAA
  • SizePrice
Product group Antibodies
Bioss
GAA Polyclonal Antibody, APC-Cy7 ConjugatedBS-13254R-APC-CY7
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetGAA
  • SizePrice
Product group Antibodies
Bioss
GAA Polyclonal Antibody, PE-Cy5 ConjugatedBS-13254R-PE-CY5
ApplicationsWestern Blot
TargetGAA
  • SizePrice
Product group Antibodies
Bioss
GAA Polyclonal Antibody, PE-Cy7 ConjugatedBS-13254R-PE-CY7
ApplicationsWestern Blot
TargetGAA
  • SizePrice
Figure 1. Western blot analysis of GAA using anti-GAAantibody (M01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GAA antigen affinity purified polyclonal antibody (Catalog # M01548) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76KD. The expected band size for GAA is at 105KD.
Product group Antibodies
Boster Bio
Anti-GAA Antibody Picoband(r) (monoclonal, 2G7)M01548-BIOTIN
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetGAA
  • SizePrice
Figure 1. Western blot analysis of GAA using anti-GAAantibody (M01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GAA antigen affinity purified polyclonal antibody (Catalog # M01548) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76KD. The expected band size for GAA is at 105KD.
Product group Antibodies
Boster Bio
Anti-GAA Antibody Picoband(r) (monoclonal, 2G7)M01548-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetGAA
  • SizePrice
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