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Figure 1. Western blot analysis of GAA using anti-GAAantibody (M01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GAA antigen affinity purified polyclonal antibody (Catalog # M01548) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76KD. The expected band size for GAA is at 105KD.
Product group Antibodies
Boster Bio
Anti-GAA Antibody Picoband(r) (monoclonal, 2G7)M01548
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetGAA
  • SizePrice
Product group Cell Culture
Applied Biological Materials
GAA CRISPR Knockout 293T Cell LineT3006976
CategoryCell Culture
  • SizePrice
Product group Chemicals
Biosynth
NSC 45586 [6300-44-3]GAA30044
CAS Number6300-44-3
Molecular Weight413.4 g/mol
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Product group Chemicals
Biosynth
Bis(4-chlorobutyl) Ether [6334-96-9]GAA33496
CAS Number6334-96-9
Molecular Weight199.12 g/mol
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Product group Chemicals
Biosynth
Bis(2-chlorophenyl)methanol [6335-15-5] [6335-15-5]GAA33515
CAS Number6335-15-5
Molecular Weight253.12 g/mol
  • SizePrice
Product group Chemicals
Biosynth
(4-methylpiperazinyl)(phenylamino)methane-1-thione [6336-69-2]GAA33669
CAS Number6336-69-2
  • SizePrice
Product group Chemicals
Biosynth
1-[2-(2-Aminoethoxy)ethoxy]butane [6338-53-0] [6338-53-0]GAA33853
CAS Number6338-53-0
Molecular Weight161.24 g/mol
  • SizePrice
Product group Chemicals
Biosynth
4-[(4-cyanophenyl)disulfanyl]benzonitrile [6339-51-1]GAA33951
CAS Number6339-51-1
Molecular Weight268.36 g/mol
  • SizePrice
Product group Chemicals
Biosynth
2-[(Pyridin-3-yl)amino]acetic acid hydrochloride [6345-28-4] [6345-28-4]GAA34528
CAS Number6345-28-4
Molecular Weight188.61 g/mol
  • SizePrice
Product group Chemicals
Biosynth
(4-methoxyphenyl)-N-(2-phenylethyl)formamideGAA34607
  • SizePrice
Product group Chemicals
Biosynth
Acid yellow 11 [6359-82-6]GAA35982
CAS Number6359-82-6
Molecular Weight381.36 g/mol
  • SizePrice
Product group Chemicals
Biosynth
tert-Butyl 2-azidoacetate [6367-36-8] [6367-36-8]GAA36736
CAS Number6367-36-8
Molecular Weight157.17 g/mol
  • SizePrice
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