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Figure 1. Western blot analysis of GCLM using anti-GCLM antibody (A02948-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLM antigen affinity purified polyclonal antibody (Catalog # A02948-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLM at approximately 31 kDa. The expected band size for GCLM is at 31 kDa.
Product group Antibodies
Boster Bio
Anti-GCLM Antibody Picoband(r)A02948-2-PE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetGCLM
  • SizePrice
Figure 1. Western blot analysis of GCLM using anti-GCLM antibody (A02948-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLM antigen affinity purified polyclonal antibody (Catalog # A02948-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLM at approximately 31 kDa. The expected band size for GCLM is at 31 kDa.
Product group Antibodies
References
Boster Bio
Anti-GCLM Antibody Picoband(r)A02948-2
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetGCLM
  • SizePrice
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-GCLM Monoclonal AntibodyM02948-30UL
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGCLM
  • SizePrice
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Product group Antibodies
References
Boster Bio
Anti-GCLM Monoclonal AntibodyM02948
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGCLM
  • SizePrice
Mouse GCLM ELISA Kit
Product group Assays
Antibodies.com
Mouse GCLM ELISA KitA7176
Assay Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
ReactivityMouse
  • SizePrice
Rat GCLM ELISA Kit
Product group Assays
Antibodies.com
Rat GCLM ELISA KitA7394
Assay Sample TypeTissue homogenates and other biological fluids.
ReactivityRat
  • SizePrice
Human GCLM ELISA Kit
Product group Assays
Antibodies.com
Human GCLM ELISA KitA76600
Assay Sample TypePlasma, tissue homogenates and other biological fluids.
ReactivityHuman
  • SizePrice
Rat GCLM ELISA Kit
Product group Assays
Antibodies.com
Rat GCLM ELISA KitA79366
Assay Sample TypePlasma, tissue homogenates and other biological fluids.
ReactivityRat
  • SizePrice
Anti-GCLM Antibody [ARC0597]
Product group Antibodies
Antibodies.com
Anti-GCLM Antibody [ARC0597]A81176
ApplicationsWestern Blot
ReactivityHuman
  • SizePrice
Product group Antibodies
MedChem Express
GCLM Antibody (YA1407, Rabbit)HY-P81662
TargetGCLM
  • SizePrice
Product group Antibodies
MedChem Express
GCLM Antibody (YA6249, Rabbit)HY-P86557
TargetGCLM
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GCLM AntibodyLS-C806586
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Porcine, Rat
TargetGCLM
  • SizePrice
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