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Search results: GPCR

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Western blot analysis of GPCR LGR8 using anti-GPCR LGR8 antibody (A04848-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SHG-44 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR LGR8 antigen affinity purified polyclonal antibody (Catalog # A04848-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR LGR8 at approximately 86KD. The expected band size for GPCR LGR8 is at 86KD.
Product group Antibodies
Boster Bio
Western blot analysis of GPCR GPR40 using anti-GPCR GPR40 antibody (A01349-2).
Product group Antibodies
Boster Bio
Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
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