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Search results: GRHL2

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Anti-GRHL2 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsELISA
  • SizePrice
Product group Antibodies
Abbexa
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsELISA
  • SizePrice
Product group Proteins / Signaling Molecules
Atlas Antibodies
Protein IDQ6ISB3
  • SizePrice
Product group Proteins / Signaling Molecules
Atlas Antibodies
Protein IDQ6ISB3
  • SizePrice
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetGRHL2
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Product group Antibodies
Cusabio
ReactivityHuman
TargetGRHL2
  • SizePrice
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetGRHL2
  • SizePrice
Figure 1. Western blot analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRHL2 antigen affinity purified polyclonal antibody (Catalog # A04120-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRHL2 at approximately 71 kDa. The expected band size for GRHL2 is at 71 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetGRHL2
  • SizePrice
Figure 1. Western blot analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRHL2 antigen affinity purified polyclonal antibody (Catalog # A04120-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRHL2 at approximately 71 kDa. The expected band size for GRHL2 is at 71 kDa.
Product group Antibodies
Boster Bio
Anti-GRHL2 Antibody Picoband(r)A04120-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetGRHL2
  • SizePrice
Figure 1. Western blot analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRHL2 antigen affinity purified polyclonal antibody (Catalog # A04120-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRHL2 at approximately 71 kDa. The expected band size for GRHL2 is at 71 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetGRHL2
  • SizePrice

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