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Search results: GSK3A

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Western blot (WB) analyzes of GSK3alpha pAb in extracts from CV-1 cells.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGSK3A
  • SizePrice
Western blot analysis of GSK3A (arrow) using GSK3A Antibody (C-term) (Cat.#TA325026). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GSK3A gene (Lane 2) (Origene Technologies).
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetGSK3A
  • SizePrice
WB: The 3T3 cell lysate derived from conditioned culture medium was resolved onto 10% SDSPAGE, transferred onto NC membrane, and followed by an immunoblotting with Rabbit anti-GSK-3 alpha (pS21) antibody at 1:500 (lane 1). Lane 2 was pre-incubated with immunizing peptide
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGSK3A
  • SizePrice
Western blot analysis of extracts from K562/HepG2/RAW264.7 cells, using GSK3 alpha (Ab-21) Antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGSK3A
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGSK3A
  • SizePrice
WB: The 3T3 cell lysate was resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with Rabbit anti-GSK-3 alpha antibody at 1:500 (lane 1). Lane 2 was preincubated with immunizing peptide
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGSK3A
  • SizePrice
Western blot analysis of extracts of various cell lines, using GSK3alpha antibody (TA376815) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 10s.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetGSK3A
  • SizePrice
Western blot analysis of extracts from normal (control) and GSK3alpha knockout (KO) HeLa cells, using GSK3alpha antibody (18655) at 1:500 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 5s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGSK3A
  • SizePrice
Western blot analysis of extracts of 293 and HeLa cells, using Phospho-GSK3alpha-S21 antibody (TA376817) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetGSK3A
  • SizePrice
Western blot analysis of GSK3 alpha expression in ovarycancer whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGSK3A
  • SizePrice
Western blot analysis of GSK3 lpha/beta expression in TNF-a treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGSK3A
  • SizePrice
Western blot analysis of GSK3 alpha phosphorylation expression in ovarycancer whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGSK3A
  • SizePrice