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Search results: H3K9Me3

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Anti-H3K9me3S10p polyclonal antibody
Product group Antibodies
BPS Bioscience
ApplicationsDot Blot, ImmunoFluorescence, Western Blot
ReactivityHuman
  • SizePrice
Product group Assays
Porvair Sciences
  • SizePrice
GTX33602 ELISA Image
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
GTX60327 ChIP assay Image
Product group Antibodies
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
  • SizePrice
ChIP assays were performed using HeLa cells treated with colcemid, the antibody against H3K9me3S10p (bs-53072R) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 10,000 cells per IP. A titration of the antibody consisting of 1, 5, and 10 μl per ChIP experiment was analyzed. Additionally, the same titration was analyzed after incubation of the antibody with 5 nmol blocking peptide (cat. No. sp-128-050) for 1 hour at room temperature. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes GAPDH and c-fos and for the heterochromatin marker Sat2. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
Bioss Antibodies
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
Abnova
  • SizePrice
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, ChIP Chromatin ImmunoPrecipitation
ReactivityHuman, Mouse
TargetH3C1
  • SizePrice
WB using the antibody against H3K9me3 diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityDrosophila, Human, Mouse
TargetH3C1
  • SizePrice
WB analysis was performed on h istone extracts from HeLa cells ( 15 ug, lane 1) and on recombinant H3 (1 ug, Lane 2) using the antibody against H3K9me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Product group Antibodies
OriGene
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetH3C1
  • SizePrice
Product group Antibodies
Cusabio
ORC6 AntibodyCSB-PA893418ESR2HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
Product group Antibodies
Cusabio
ORC4 AntibodyCSB-PA017234ESR1HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice