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Search results: HAUS8

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Product group Antibodies
Biorbyt
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
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Product group Proteins / Signaling Molecules
Biorbyt
Protein IDQ9BT25
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Western blot analysis of extracts of various cell lines, using HAUS8 antibody (TA376930) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetHAUS8
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WB Suggested Anti-HAUS8 Antibody Titration: 0.125 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate
Product group Antibodies
OriGene
ApplicationsWestern Blot
TargetHAUS8
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetHAUS8
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Anti-HICE1 in Western Blot using Rockland Immunochemicals Anti-HICE1 Antibody shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HICE1. In right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10,000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
TargetHAUS8
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Western Blot of Rabbit Anti-Hice1 pS70 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35microg per lane. Primary antibody: Hice1 pS70 antibody at 0.5microg/mL for overnight at 4°C. Secondary antibody: IRDye800™ Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1 pS70.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
TargetHAUS8
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Western Blot of Rabbit Anti-Hice1 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 microg per lane. Primary antibody: Hice1 antibody at 0.5 microg/mL for overnight at 4°C. Secondary antibody: IRDye800™ Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1. Other band(s): none.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
TargetHAUS8
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WB to detect cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image), using TA307936 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetHAUS8
  • SizePrice
WB of Rabbit Anti-Hice1 pS70 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 pS70 antibody at 0.5 ug/mL for overnight at 4°C. Secondary antibody: IRDye800™ Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10,000 for 45 min at RT.
Product group Antibodies
OriGene
ApplicationsWestern Blot
TargetHAUS8
  • SizePrice
Anti-HICE1 in WB using Immunochemicals Anti-HICE1 Antibody shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HICE1. In right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10,000. Molecular weight estimation was made by comparison by prestained MW markers.
Product group Antibodies
OriGene
ApplicationsWestern Blot
TargetHAUS8
  • SizePrice