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Search results: HOOK3

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Figure 1. Western blot analysis of HOOK3 using anti-HOOK3 antibody (A07701-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOOK3 antigen affinity purified polyclonal antibody (Catalog # A07701-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOOK3 at approximately 83 kDa. The expected band size for HOOK3 is at 83 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHOOK3
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Product group Molecular Biology
Abbexa
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Product group Molecular Biology
Abbexa
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Western blot analysis of HOOK3 in mouse brain tissue lysate with HOOK3 antibody at 1 microg/ml.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetHOOK3
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Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse
TargetHOOK3
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Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse
TargetHOOK3
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Product group Cell Culture
Applied Biological Materials
CategoryCell Culture
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Product group Molecular Biology
MedChem Express
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Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
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Product group Antibodies
Biorbyt
HOOK3ORB1281914
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetHOOK3
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Product group Antibodies
Biorbyt
HOOK3ORB1275033
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetHOOK3
  • SizePrice
Product group Antibodies
Biorbyt
HOOK3 AntibodyORB1239706
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetHOOK3
  • SizePrice