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Search results: HOXC10

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Figure 1. Western blot analysis of HOXC10 using anti-HOXC10 antibody (A09018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXC10 antigen affinity purified polyclonal antibody (Catalog # A09018-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXC10 at approximately 48KD. The expected band size for HOXC10 is at 38KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetHOXC10
  • SizePrice
Figure 1. Western blot analysis of HOXC10 using anti-HOXC10 antibody (A09018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXC10 antigen affinity purified polyclonal antibody (Catalog # A09018-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXC10 at approximately 48KD. The expected band size for HOXC10 is at 38KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetHOXC10
  • SizePrice
Product group Molecular Biology
Abbexa
  • SizePrice
Product group Molecular Biology
Abbexa
  • SizePrice
Western blot analysis of lysates from Hela cell line, mouse skeletal muscle and mouse cerebellum tissue lysate (from left to right), using Mouse Hoxc10 Antibody (C-term). A09018-2 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityMouse
TargetHoxc10
  • SizePrice
Western blot analysis of lysates from Hela cell line, mouse skeletal muscle and mouse cerebellum tissue lysate (from left to right), using Mouse Hoxc10 Antibody (C-term). A09018-2 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityMouse
TargetHoxc10
  • SizePrice
Product group Molecular Biology
Sino Biological
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Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation
ReactivityHuman
TargetHOXC10
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Monkey, Mouse
TargetHOXC10
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • SizePrice