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Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityRat
TargetMap4k1
  • SizePrice
Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 91 kDa. The expected band size for MAP4K1/HPK1 is at 91 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMAP4K1
  • SizePrice

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