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Search results: HRH3

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Product group Assays
DLdevelop
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Product group Assays
DLdevelop
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Product group Assays
DLdevelop
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Product group Assays
DLdevelop
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Product group Assays
DLdevelop
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoPrecipitation
ReactivityHuman, Mouse, Rat
TargetHRH3
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHRH3
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Western blot analysis of HRH3 using anti-HRH3 antibody (PA1204). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates Lane 2: human SHG-44 whole cell lysates Lane 3: mouse RAW246.7 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRH3 antigen affinity purified polyclonal antibody (Catalog # PA1204) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRH3 at approximately 49KD. The expected band size for HRH3 is at 49KD.
Product group Antibodies
Boster Bio
Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Equine, Guinea Pig, Hamster, Human, Monkey, Mouse, Rat
TargetHRH3
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetHRH3
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Monkey
TargetHRH3
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetHRH3
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