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Search results: HSPD1

265 to 276 of 1.327 products
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Product group Antibodies
MyBioSource
HSPD1 AntibodyMBS7123813
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Product group Antibodies
MyBioSource
HSPD1 AntibodyMBS7123814
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
ReactivityGuinea Pig, Human, Rabbit, Rat
TargetHSPD1
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Rabbit, Rat, Sheep, Yeast, Zebra Fish
TargetHspd1
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Product group Antibodies
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Porcine
TargetHSPD1
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Product group Antibodies
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityBovine, Canine, Chicken, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
TargetHSPD1
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Product group Antibodies
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityChicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Rat
TargetHSPD1
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityBovine, Canine, Chicken, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
TargetHSPD1
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Figure 1. Western blot analysis of HSP60 using anti-HSP60 antibody (MA1049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSP60 antigen affinity purified monoclonal antibody (Catalog # MA1049) at 2 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSP60 at approximately 65 kDa. The expected band size for HSP60 is at 65 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Monkey, Mouse, Rat
TargetHSPD1
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHSPD1
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Product group Antibodies
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetHSPD1
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Anti-Hsp60 Picoband antibody, PB9337, IHC(P) IHC(P): Mouse Intestine Tissue
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHSPD1
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