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Search results: HSPD1

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Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetHSPD1
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetHSPD1
  • SizePrice
Anti-HSP60 Antibody [rHSPD1/6495] - BSA and Azide free
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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Anti-HSP60 Antibody [rHSPD1/6497] - BSA and Azide free
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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Product group Proteins / Signaling Molecules
Abbexa
ApplicationsWestern Blot, Other Application
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Product group Proteins / Signaling Molecules
Abbexa
ApplicationsWestern Blot, Other Application
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Product group Proteins / Signaling Molecules
Abbexa
ApplicationsWestern Blot, Other Application
  • SizePrice
Product group Proteins / Signaling Molecules
Abbexa
ApplicationsWestern Blot, Other Application
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Product group Proteins / Signaling Molecules
Abbexa
ApplicationsOther Application
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Product group Proteins / Signaling Molecules
Abbexa
ApplicationsOther Application
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Figure 1. Western blot analysis of HSP60 using anti-HSP60 antibody (MA1049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSP60 antigen affinity purified monoclonal antibody (Catalog # MA1049) at 2 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSP60 at approximately 65 kDa. The expected band size for HSP60 is at 65 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetHSPD1
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Product group Proteins / Signaling Molecules
Aviva Systems Biology
ApplicationsWestern Blot
Protein IDP63038
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