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Search results: HSPD1

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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHSPD1
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Product group Proteins / Signaling Molecules
Abbexa
ApplicationsOther Application
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Product group Cell Culture
Applied Biological Materials
CategoryCell Culture
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Product group Molecular Biology
MedChem Express
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Product group Antibodies
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Porcine, Rat
TargetHSPD1
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Anti-HSP60 Antibody [rHSPD1/6495]
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityChicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Rat
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Anti-HSP60 Antibody [rHSPD1/6497]
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
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Product group Assays
Biomatik
Assay Sample TypeSerum, Plasma, Biological Fluids
ReactivityHuman
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Figure 1. Western blot analysis of HSPD1 using anti-HSPD1 antibody (M01280-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human A549 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human SW620 whole cell lysates Lane 5: human U-937 whole cell lysates Lane 6: human HepG2 whole cell lysates Lane 7: rat RH35 whole cell lysates Lane 8: mouse RAW246.7 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPD1 antigen affinity purified monoclonal antibody (Catalog # M01280-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPD1 at approximately 60KD. The expected band size for HSPD1 is at 60KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHSPD1
  • SizePrice
Figure 1. Western blot analysis of HSPD1 using anti-HSPD1 antibody (M01280-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human A549 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human SW620 whole cell lysates Lane 5: human U-937 whole cell lysates Lane 6: human HepG2 whole cell lysates Lane 7: rat RH35 whole cell lysates Lane 8: mouse RAW246.7 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPD1 antigen affinity purified monoclonal antibody (Catalog # M01280-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPD1 at approximately 60KD. The expected band size for HSPD1 is at 60KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHSPD1
  • SizePrice
Figure 1. Western blot analysis of HSPD1 using anti-HSPD1 antibody (M01280-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human A549 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human SW620 whole cell lysates Lane 5: human U-937 whole cell lysates Lane 6: human HepG2 whole cell lysates Lane 7: rat RH35 whole cell lysates Lane 8: mouse RAW246.7 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPD1 antigen affinity purified monoclonal antibody (Catalog # M01280-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPD1 at approximately 60KD. The expected band size for HSPD1 is at 60KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHSPD1
  • SizePrice
Figure 1. Western blot analysis of HSPD1 using anti-HSPD1 antibody (M01280-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human A549 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human SW620 whole cell lysates Lane 5: human U-937 whole cell lysates Lane 6: human HepG2 whole cell lysates Lane 7: rat RH35 whole cell lysates Lane 8: mouse RAW246.7 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPD1 antigen affinity purified monoclonal antibody (Catalog # M01280-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPD1 at approximately 60KD. The expected band size for HSPD1 is at 60KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHSPD1
  • SizePrice