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Search results: IDE

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Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetIDE
  • SizePrice
Product group Antibodies
Bioss
ApplicationsImmunoFluorescence
ReactivityHuman
TargetIDE
  • SizePrice
Product group Proteins / Signaling Molecules
Abbexa
ApplicationsWestern Blot, Other Application
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsELISA
  • SizePrice
Product group Antibodies
Abbexa
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsELISA
  • SizePrice
Figure 1. Western blot analysis of IDE using anti-IDE antibody (M01358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified monoclonal antibody (M01358) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDE at approximately 118 kDa. The expected band size for IDE is at 118 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetIDE
  • SizePrice
Figure 1. Western blot analysis of IDE using anti-IDE antibody (M01358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified monoclonal antibody (M01358) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDE at approximately 118 kDa. The expected band size for IDE is at 118 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetIDE
  • SizePrice
Product group Antibodies
Bioss
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetIDE
  • SizePrice
Product group Antibodies
Bioss
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetIDE
  • SizePrice
Product group Antibodies
Bioss
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetIDE
  • SizePrice
Product group Antibodies
Bioss
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetIDE
  • SizePrice

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