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Search results: IKK alpha

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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
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Western blot analysis of IKK alpha expression in Daudi cell lysate (M01918-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHUK monoclonal antibody (Catalog # M01918-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHUK
Product group Antibodies
Boster Bio
Product group Antibodies
Biorbyt
IKK alphaORB5508
ApplicationsELISA
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetCHUK
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetCHUK
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetCHUK
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetCHUK
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
TargetCHUK
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetCHUK
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetCHUK
  • SizePrice