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Search results: JNK1 MAPK8

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Product group DNA / RNA / Vectors
OriGene
CategorycDNA
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Product group DNA / RNA / Vectors
OriGene
CategorycDNA
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Western blot analysis of JNK1/JNK3 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetMAPK8
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Figure 2. Immunohistochemistry validation of MAPK8 using Anti-JNK1/2/3 (T183/Y185) MAPK8 Antibody (A02608T183Y185). Immunohistochemistry (IHC) analyzes of JNK1/2/3 (T183/Y185) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50. For more protocol information of IHC
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityMouse, Rat
TargetMAPK8
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Figure 10. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1). Immunohistochemical analysis of mouse brain tissue. A02608Y185-1 was diluted at 1:200 (4°C
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK8
  • SizePrice
Figure 1. Western blot analysis of JNK1/2/3 using anti-JNK1/2/3 antibody (M02608-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK1/2/3 antigen affinity purified monoclonal antibody (Catalog # M02608-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JNK1/2/3 at approximately 40, 48 kDa. The expected band size for JNK1/2/3 is at 48 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK8
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Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
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Circular map for RC217546L4
Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
  • SizePrice
Product group DNA / RNA / Vectors
OriGene
CategoryPlasmid / vector
  • SizePrice