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Search results: JUP

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Gel: 6%SDS-PAGE Lysate: 40 microg Lane 1-4: Mouse heart tissue Hela cells HUVEC cells mouse skin tissue Primary antibody: TA350108 (JUP Antibody) at dilution 1/1150 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetJUP
  • SizePrice
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA321450 (JUP Antibody) at dilution 1/20 (Original magnification: x200)
Product group Antibodies
OriGene
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetJUP
  • SizePrice
Gel: 10%SDS-PAGE Lysate: 50 microg Lane 1-2: A549 cells human fetal brain tissue Primary antibody: TA321451 (JUP Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 2 minutes
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetJUP
  • SizePrice
Western blot analysis of extracts of various cell lines, using JUP antibody.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse
TargetJUP
  • SizePrice
Western blot analysis of JUP Antibody (C-term) (Cat. #TA325089) in MCF-7 cell line lysates (35ug/lane). JUP (arrow) was detected using the purified Pab.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Porcine, Rat
TargetJUP
  • SizePrice
Western blot (WB) analysis of Catenin-? antibody at 1/500 dilution Lane 1:Hela cell lysate Lane 2:NIH-3T3 cell lysate Lane 3:Rat brain tissue lysate
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetJUP
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
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Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
  • SizePrice
Figure 1. Western blot analysis of JUP using anti-JUP antibody (PA1117-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUP antigen affinity purified polyclonal antibody (Catalog # PA1117-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JUP at approximately 82 kDa. The expected band size for JUP is at 82 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHamster, Human
TargetJUP
  • SizePrice
Figure 1. Western blot analysis of JUP using anti-JUP antibody (PA1117-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUP antigen affinity purified polyclonal antibody (Catalog # PA1117-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JUP at approximately 82 kDa. The expected band size for JUP is at 82 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHamster, Human
TargetJUP
  • SizePrice
Figure 1. Western blot analysis of JUP using anti-JUP antibody (PA1117-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUP antigen affinity purified polyclonal antibody (Catalog # PA1117-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JUP at approximately 82 kDa. The expected band size for JUP is at 82 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHamster, Human
TargetJUP
  • SizePrice
Figure 1. Western blot analysis of JUP using anti-JUP antibody (PA1117-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUP antigen affinity purified polyclonal antibody (Catalog # PA1117-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JUP at approximately 82 kDa. The expected band size for JUP is at 82 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHamster, Human
TargetJUP
  • SizePrice