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Figure 1. Western blot analysis of LCP1 using anti-LCP1 antibody (A03361). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCP1 antigen affinity purified polyclonal antibody (Catalog # A03361) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCP1 at approximately 70 kDa. The expected band size for LCP1 is at 70 kDa.
Product group Antibodies
References
Boster Bio
Anti-Plastin L/LCP1 Antibody Picoband(r)A03361
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetLCP1
  • SizePrice
Product group Molecular Biology
Abbexa
Mouse LCP1 shRNA PlasmidABX972107
  • SizePrice
Product group Molecular Biology
Abbexa
Human LCP1 shRNA PlasmidABX952667
  • SizePrice
Product group Assays
Biomatik
Human Plastin-2 (Lcp1) ELISA KitEKC35041
Assay Sample Typeserum, plasma, tissue homogenates
  • SizePrice
Product group Assays
Biomatik
Mouse Plastin-2 (Lcp1) ELISA KitEKC37590
Assay Sample Typeserum, plasma, tissue homogenates, cell lysates
  • SizePrice
Product group Antibodies
Abbexa
Lymphocyte Cytosolic Protein 1 (LCP1) AntibodyABX213461
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Abbexa
Lymphocyte Cytosolic Protein 1 (LCP1) AntibodyABX213462
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Abbexa
Lymphocyte Cytosolic Protein 1 (LCP1) AntibodyABX004260
ApplicationsImmunoPrecipitation, Western Blot
  • SizePrice
Product group Antibodies
Abbexa
Lymphocyte Cytosolic Protein 1 (LCP1) AntibodyABX030593
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Assays
Abbexa
Human Plastin-2 (LCP1) ELISA KitABX152177
  • SizePrice
Product group Antibodies
Abbexa
Lymphocyte Cytosolic Protein 1 (LCP1) AntibodyABX302720
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Abbexa
Lymphocyte Cytosolic Protein 1 (LCP1) AntibodyABX304334
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
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