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Search results: LOX CRISPR

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Figure 1. Western blot analysis of LOX using anti-LOX antibody (M00575). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified monoclonal antibody (Catalog # M00575) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetLOX
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Human 15-LOX-2 ELISA Kit
Product group Assays
Antibodies.com
Assay Sample TypePlasma, tissue homogenates and other biological fluids.
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Human e-LOX-3 ELISA Kit
Product group Assays
Antibodies.com
Assay Sample TypePlasma, tissue homogenates and other biological fluids.
  • SizePrice
Human e-LOX-3 ELISA Kit
Product group Assays
Antibodies.com
Assay Sample TypeTissue homogenates, cell lysates and other biological fluids.
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Product group Chemicals
MedChem Express
Molecular Weight361.85
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Product group Chemicals
MedChem Express
Molecular Weight383.83
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Product group Proteins / Signaling Molecules
MedChem Express
Protein IDG7PJT2
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Product group Proteins / Signaling Molecules
MedChem Express
Protein IDQ9EQ09
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Product group Proteins / Signaling Molecules
MedChem Express
Protein IDO70156
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LOX-1 Protein, Mouse, Recombinant (His)
Product group Chemicals
TargetMol Chemicals
Molecular Weight36.1 kDa (predicted). Due to glycosylation, the protein migrates to 55-63 kDa based on Tris-Bis PAGE result.
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Product group Proteins / Signaling Molecules
MedChem Express
Protein IDO70156
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Product group Antibodies
Abbexa
ApplicationsELISA
  • SizePrice