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Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PA1377-BIOTIN
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PA1377-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PA1377-CY3
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PA1377-DYLIGHT488
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PA1377-DYLIGHT550
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PA1377-DYLIGHT594
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PA1377-FITC
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PA1377-HRP
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PA1377-IFLUOR647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HELA whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat NRK whole cell lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39KD. The expected band size for MAP2K3 is at 39KD.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PA1377-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MEK3 using anti-MEK3 antibody (PB9763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK3 antigen affinity purified polyclonal antibody (Catalog # PB9763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK3 at approximately 40 kDa. The expected band size for MEK3 is at 40 kDa.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PB9763-BIOTIN
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MEK3 using anti-MEK3 antibody (PB9763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK3 antigen affinity purified polyclonal antibody (Catalog # PB9763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK3 at approximately 40 kDa. The expected band size for MEK3 is at 40 kDa.
Product group Antibodies
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PB9763-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
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