More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/

Search results: MAP2K1 MEK1

145 to 156 of 3.128 products
Sort by
Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
Product group Antibodies
Boster Bio
Anti-MEK1/MAP2K1 Antibody Picoband(r)A00292-IFLUOR647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetMAP2K1
  • SizePrice
Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
Product group Antibodies
Boster Bio
Anti-MEK1/MAP2K1 Antibody Picoband(r)A00292-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetMAP2K1
  • SizePrice
Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
Product group Antibodies
References
Boster Bio
Anti-MEK1/MAP2K1 Antibody Picoband(r)A00292
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetMAP2K1
  • SizePrice
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Product group Antibodies
Atlas Antibodies
Anti-MAP2K1 AntibodyHPA026430
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMAP2K1
  • SizePrice
Figure 1. Western blotting validation for Anti-MEK1/2 (S211) MAP2K1 Antibody A00292S211 Western blot (WB) analysis of MEK1/2 (S211) pAb at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:Panc1 whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug) Lane5:Hela whole cell lysate(20ug) Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Product group Antibodies
Boster Bio
Anti-MEK1/2 (S211) MAP2K1 AntibodyA00292S211
ApplicationsWestern Blot
TargetMAP2K1
  • SizePrice
Figure 2. Immunohistochemistry validation of MAP2K1 using Anti-MEK1 (phospho-T292) MAP2K1 Antibody (A00292T292). Immunohistochemistry (IHC) analyzes of p-MEK1 (T292) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. For more protocol information of IHC
Product group Antibodies
Boster Bio
Anti-MEK1 (phospho-T292) MAP2K1 AntibodyA00292T292
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetMAP2K1
  • SizePrice
Figure 2. Immunocytochemistry staining of MAP2K1 using Anti-MEK1 (Phospho-S298) MAP2K1 Antibody (A00292S298-1). ICC staining Phospho-MEK1 (S298) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
Product group Antibodies
Boster Bio
Anti-MEK1 (Phospho-S298) MAP2K1 AntibodyA00292S298-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 AntibodyLS-C808837
ApplicationsWestern Blot, ELISA
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 AntibodyLS-C808672
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 AntibodyLS-C808674
ApplicationsWestern Blot, ELISA
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 AntibodyLS-C813175
ApplicationsWestern Blot, ELISA
TargetMAP2K1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 AntibodyLS-C836155
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
TargetMAP2K1
  • SizePrice
1112131415

Didn't find what you were looking for?

Search through our product groups to find the right product

Back to overview
AntibodiesChemicalsProteins / Signaling MoleculesAssaysDNA / RNA / VectorsCell CultureGenome Editing and EngineeringMolecular BiologyLysates / ExtractsExpressionLabware / AccesoriesBody ProductsTechnique Specific ProductsArrays / SlidesDiagnosticBiologicalsMicrobiologyPromotions