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Search results: MAP2K1 MEK1

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Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit pAb (TA378244) at 1:1000 dilution or MEK1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30℃ for 1 hour.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 1s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378244
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of lysates from C6 cells, using Phospho-MEK1-pS221 Rabbit pAb (TA378245) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 60s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378245
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western Blot: MEK1 Antibody [TA336999] - Analysis of MEK1/MEK2 phosphorylation in A375 whole cell lysate. Lane A shows phospho MEK1/MEK2 (upper band), and the weakly detected nonphosphorylated form (lower band), using MEK1 phospho antibody at 3 ug/ml. Lan
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA336999
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Drosophila, Human, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1/2 in human brain tissue lysate with MEK1/2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA349182
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA349388 (MAP2K1 Antibody) at dilution 1/25 (Original magnification: x200)
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA349388
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
WB: The whole cell lysates derived from 3T3 were immunoblotted by Rabbit anti-MEK1/2 antibody at 1:1000.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA354399
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MAP2K1 (arrow) using rabbit polyclonal MAP2K1 Antibody (S217) (Cat.#TA302208).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K1 gene (Lane 2) (Origene Technologies).
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA302208
ApplicationsWestern Blot
ReactivityChicken, Drosophila, Hamster, Human, Mouse, Rabbit, Rat, Xenopus
TargetMAP2K1
  • SizePrice
MAP2K1 Antibody (pT291) (Cat.# TA302209) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MAP2K1 antibody detected the MAP2K1 protein (arrow).
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA302209
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rabbit, Rat
TargetMAP2K1
  • SizePrice
Western blot of human T47D cells showing specific immunolabeling of the ~45k MEK 1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: I>> Ptase) The blot is identical to the control except that it was incubated in I>> Ptase (1200 units for 30 min) before being exposed to the MEK 1 Thr386 antibody. The immunolabeling of MEK 1 is completely eliminated by I>> Ptase.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA309168
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetMAP2K1
  • SizePrice
Gel: 10%SDS-PAGE Lysate: 40 microg Lane: Mouse brain tissue Primary antibody: TA321522 (MAP2K1 Antibody) at dilution 1/450 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA321522
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1 expression in K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA325632
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1/2 expression in PMA treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA325633
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
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