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Search results: MAP2K1 MEK1

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Detection of endogenous MEK1/2 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MEK1/2-9G3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
MAP2K1 Elisa with 3E6 Capture (TA600334) and 7B1 Detection (TA700334) Antibodies. Substrate used: Recombinant Human MAP2K1 (TP318460)
Product group Antibodies
OriGene
ApplicationsELISA
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
MAP2K1 Elisa with 1F9 Capture (TA600338) and 2B2 Detection (TA700335) Antibodies. Substrate used: Recombinant Human MAP2K1 (TP318460)
Product group Antibodies
OriGene
ApplicationsELISA
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Anti-MEK1 antibody, PA1376, Western blotting Lane 1: Rat Skeletal Muscle Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: CEM Cell Lysate Lane 4: COLO20 Cell Lysate
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K1
  • SizePrice
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMAP2K1
  • SizePrice
Figure 1. Western blot analysis of MEK1-2 using anti-MEK1-2 antibody (M00292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1-2 antigen affinity purified monoclonal antibody (Catalog # M00292-1) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1-2 at approximately 43 kDa. The expected band size for MEK1-2 is at 43 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Assays
RayBiotech
ReactivityMouse
  • SizePrice
Product group Antibodies
Assay Genie
ReactivityHuman
TargetMAP2K1
  • SizePrice
Western blot analysis of Phospho-MEK1 (S298) expression in (1) HeLa cell treated with LP lysate; (2) HeLa cell lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice

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