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Search results: MAP2K1 MEK1

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Product group Antibodies
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetLAMTOR3
  • SizePrice
Mp1 Protein, Human, Recombinant (His)
Product group Chemicals
TargetMol Chemicals
Molecular Weight15 KDa (reducing condition)
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsNeutralisation/Blocking
  • SizePrice
IHC-P analysis of human brain tissue using GTX31568 MP1 antibody. Working concentration : 5 microg/ml
Product group Antibodies
GeneTex
MP1 antibodyGTX31568
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetLAMTOR3
  • SizePrice
3microg Human MP1 protein (GTX67989-pro) by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Product group Molecular Biology
GeneTex
  • SizePrice
Product group Chemicals
Biorbyt
RO4987655ORB181310
CAS Number874101-00-5
Molecular Weight565.28
  • SizePrice
Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetMAP2K1
  • SizePrice
Western Blot Positive WB detected in: LO2 whole cell lysate, Mouse heart tissue, Mouse kidney tissue All lanes: MAP2K1 antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 41 kDa Observed band size: 44 kDa
Product group Antibodies
Cusabio
MAP2K1 AntibodyCSB-PA013409LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP2K1
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Product group Antibodies
Assay Genie
TargetMAP2K1
  • SizePrice
Product group Antibodies
Assay Genie
TargetMAP2K1
  • SizePrice
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetMAP2K1
  • SizePrice
Product group Antibodies
Cusabio
ReactivityHuman
TargetMAP2K1
  • SizePrice

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