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Search results: MAP2K5 MEK5

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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Western blot of rat testes lysate showing phospho-specific immunolabeling of the ~49k MEK5 protein phosphorylated at Ser 311 and Thr 315 using MEK5 (phospho Ser311/Thr315) antibody (GTX82655).
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityRat
TargetMap2k5
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WB analysis of extracts from rat and mouse brain tissue using GTX50845 MEK5 (phospho Ser311/ Thr315) antibody.
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityMouse, Rat
TargetMap2k5
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Western blot analysis of MEK5 expression in HeLa cell lysate (M03980). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K5 monoclonal antibody (Catalog # M03980) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K5
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetMAP2K5
Product group Molecular Biology
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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