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Search results: MAP2K6 MEK6 MKK6

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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetMAP2K6
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetMAP2K6
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetMAP2K6
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetMAP2K6
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetMAP2K6
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetMAP2K6
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Figure 1. Western blot analysis of MKK6/MAP2K6 using anti-MKK6/MAP2K6 antibody (A02011). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKK6/MAP2K6 antigen affinity purified polyclonal antibody (Catalog # A02011) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKK6/MAP2K6 at approximately 38 kDa. The expected band size for MKK6/MAP2K6 is at 38 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetMAP2K6
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Anti-MEK6 antibody, PA1921, Western blotting Lane 1: Rat Ovary Tissue Lysate Lane 2: Rat Spleen Tissue Lysate Lane 3: Rat Brain Tissue Lysate Lane 4: Rat Heart Tissue Lysate Lane 5: NIH3I3 Cell Lysate Lane 6: HELA Cell Lysate Lane 7: JRUKAT Cell Lysate Lane 8: MCF-7 Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetMAP2K6
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetMAP2K6
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetMAP2K6
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetMAP2K6
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetMAP2K6
  • SizePrice