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Search results: MAP2K6 MEK6 MKK6

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Product group Proteins / Signaling Molecules
MedChem Express
Protein IDP52564
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Western blot analysis of MEK3/MEK6 expression in HeLa cell lysate (M02916). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 monoclonal antibody (Catalog # M02916) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K3
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Product group Antibodies
Abbexa
ApplicationsWestern Blot
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Mouse muscle lysates probed with MKK3(Ser218) + MKK6(Ser207) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetMAP2K3
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MKK6 Cell Based ELISA Kit
Product group Assays
Antibodies.com
Assay Sample TypeAdherent cells and suspension cells.
ReactivityHuman, Mouse, Rat
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Product group Antibodies
Assay Genie
ReactivityHuman
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Product group Assays
Biomatik
Assay Sample TypeResearchers plate their cell line of choice
ReactivityHuman, Mouse, Rat
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Product group Assays
Assay Genie
Assay Sample TypeAdherent Cells
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Product group Assays
Assay Genie
Assay Sample TypeAdherent Cells
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Product group Chemicals
TargetMol Chemicals
Molecular Weight37.7 kDa (predicted)
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Product group Chemicals
TargetMol Chemicals
Molecular Weight65.3 kDa (predicted); 60 kDa (reducing conditions)
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MAP2K6 Cell Based ELISA Kit
Product group Assays
Antibodies.com
Assay Sample TypeAdherent cells and suspension cells.
ReactivityHuman, Mouse, Rat
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